Thermo Fisher Scientific ROCK1 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human ROCK1.

Target Information

ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 has been identified as a Caspase-3 substrate.
ROCK1 contributes to myosin light chain (MLC) activation through two mechanisms: direct phosphorylation of MLC and phosphorylation-mediated inhibition of MLC phosphatase. This leads to increased cellular contractility, necessary for apoptotic membrane blebbing.
ROCK1 consists of an N-terminal kinase domain and a C-terminal inhibitory cysteine/histidine-rich domain within a pleckstrin-homology region. These domains are linked by a variable region containing the Rho-binding domain. During apoptosis, ROCK1 is cleaved by caspase-3 at a conserved DETD1113/G sequence, removing its inhibitory domain and resulting in constitutive kinase activity. This cleavage induces MLC phosphorylation and membrane blebbing, key events in apoptosis execution.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.