Thermo Fisher Scientific PDHA1 Monoclonal Antibody (OTI2C10), TrueMAB
인간 PDHA1 단백질을 인식하는 Mouse IgG2b 단일클론 항체로 Western blot, ICC/IF, Flow Cytometry에 적합. 고순도 친화 크로마토그래피 정제, 액상 형태로 제공. 안정적 PBS/BSA/glycerol 버퍼에 보관하며 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Flow Cytometry (Flow)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2C10 |
| Immunogen | Full length human recombinant protein of human PDHA1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.75 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Target Information
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the conversion of pyruvate to acetyl-CoA and CO₂, providing the primary link between glycolysis and the tricarboxylic acid (TCA) cycle.
The PDH complex consists of multiple copies of three enzymatic components:
- Pyruvate dehydrogenase (E1)
- Dihydrolipoamide acetyltransferase (E2)
- Lipoamide dehydrogenase (E3)
The E1 enzyme is a heterotetramer composed of two alpha and two beta subunits.
This gene encodes the E1 alpha 1 subunit containing the E1 active site and plays a critical role in PDH complex function.
Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome.
Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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