Thermo Fisher Scientific TOP2A Monoclonal Antibody (OTI10G10), TrueMAB
Human TOP2A 단백질(aa 1100-1531)에 특이적인 Mouse IgG1 단클론 항체. Western blot에서 1:2,000 희석으로 검증됨. 동결건조 형태로 제공되며 PBS/trehalose 버퍼에 보관. DNA 합성 및 분열 관련 연구에 적합.
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Applications
- Western Blot (WB)
Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI10G10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1100–1531 of human TOP2A produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step.
Recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
Topo II alpha is involved in DNA synthesis, transcription, and chromosomal segregation during mitosis.
It serves as a sensitive and specific marker for late S-, G2-, and M-phases in transformed and normal cells under developmental regulation.
Topo II alpha is also associated with tumor cell drug resistance.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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