Thermo Fisher Scientific Phospho-SRC (Tyr527) Polyclonal Antibody
SRC 단백질의 Tyr527 인산화 부위를 인식하는 rabbit polyclonal antibody. Western blot과 IHC(P)에서 검증됨. 항원 친화 크로마토그래피로 정제된 액상 항체로, 세포 신호전달 및 종양 연구에 적합. -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pTyr527 of human Src |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 20 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 50% glycerol, 0.15M NaCl, 100 µg/mL BSA |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10980114 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. It plays a key role in growth and differentiation of eukaryotic cells by associating with the cytoplasmic region of various cell surface receptors to facilitate signal transduction. SRC also contributes to cytoskeletal organization, cell cycle regulation, and apoptosis.
The SRC gene is located on chromosome 20 in humans. Its activity is regulated by phosphorylation at two tyrosine residues: Tyr416 and Tyr527. Phosphorylation at Tyr416 upregulates enzyme activity, while phosphorylation at Tyr527 decreases it. Mutations in the SRC gene may contribute to malignant progression in colon cancer. Two transcript variants encoding the same SRC protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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