Gibco™

Ham`s F-12 Nutrient Mix

Ham`s F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since자세히 알아보기

Ham`s F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Gibco™ F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham`s F-12 is modified as follows:

With
• L-glutamine
• Phenol Red

The complete formulation is available.

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations.

Gibco™ F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

사양

배양 환경CO2

세포 유형Mammalian Cells

분류Animal Origin-free

농도1 X

Culture TypeMammalian Cell Culture

형태Liquid

제품 유형Ham`s F-12 Nutrient Mix

유통 기한12 Months

멸균Sterile-filtered

첨가제 포함Glutamine, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate

첨가제 없음No HEPES

제조 품질cGMP-compliant under the ISO 13485 standard

삼투압280 - 310 mOsm/kg

pH7.1 to 7.5

제품라인Gibco™

수량500 mL

배송 조건Room Temperature

Unit SizeEach