Thermo Fisher Scientific EEF2 Recombinant Rabbit Monoclonal Antibody (JE32-57)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Immunocytochemistry (ICC/IF)

1:250

-

Flow Cytometry (Flow)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JE32-57

Immunogen

Synthetic peptide within Human EEF2 aa 809-858/858. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EEF2,uniProtId:P13639-1,ncbiNodeId:9606,antigenRange:809-858,antigenLength:858,antigenImageFileName:MA5-50830_EEF2_P13639-1_Rabbit.svg,antigenImageFileNamePDP:MA5-50830_EEF2_P13639-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 0.05% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3094130

Product Specific Information

Positive control: HeLa cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, rat spleen tissue lysate, HeLa, NIH/3T3.

Predicted band size: 95 kDa

Subcellular Location: Cytoplasm, Nucleus.

Target Information

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.