Thermo Fisher Scientific Septin 9 Polyclonal Antibody
Rabbit polyclonal antibody against human Septin 9. Validated for WB, IHC, IHC-P, and IP applications. Recommended dilutions: WB 1:2,000–1:10,000; IHC 1:100–1:500; IP 2–5 µg/mg lysate. Supplied as liquid at 0.2 mg/mL, stored at 4°C. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 100 and 150 of Human Septin 9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
The maf oncogene was identified by structural analysis of the AS42 avian transforming retrovirus genome. The Maf family is divided into two subclasses: large Mafs (vMaf, cMaf, MafB, and Nrl) and small Mafs (MafF, MafK, and MafG). Both subclasses contain leucine zipper motifs, allowing homodimerization and heterodimerization with other bZip transcription factors.
Large Mafs include an acidic transactivation domain absent in small Maf proteins. Although small Mafs lack inherent transactivation activity, they can act as positive regulators of transcription by forming active dimers or as negative regulators by recruiting repressors or forming inactive homodimers.
Human MafF, a small Maf protein of 164 amino acids, was isolated from a human myometrium cDNA library. Small Maf proteins are implicated in development, differentiation, hematopoiesis, and stress response, regulating these processes via distinct mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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