Thermo Fisher Scientific Hydromorphone Polyclonal Antibody

Applications

Tested Dilution

Publications

ELISA (ELISA)

5-10 µg/mL

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Hydromorphone(3)-BTG

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

7.28 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.8

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1090258

Product Specific Information

Working Concentration: 1.25 µg/mL. Sensitivity: 10 ng/mL Hydromorphone produces 74% inhibition in a competitive ELISA employing Hydromorphone polyclonal antibody.

Target Information

Hydromorphone Base is the hydrogenated ketone of morphine, a semi-synthetic opioid with analgesic effects. Hydromorphone selectively binds the mu-opioid receptor which is linked through G-proteins. Binding stimulates the exchange of guanosine triphosphate (GTP) for guanosine diphosphate (GDP) on the G-protein complex and interacts with and inhibits adenylate cyclase located at the inner surface of the plasma membrane. This leads to a reduction in intracellular cyclic 3,5-adenosine monophosphate (cAMP). Further, voltage-gated potassium channels are activated, thereby causing hyperpolarization and reducing neuronal excitability. In addition, the opening of voltage-gated calcium channels is inhibited, thereby leading to an inhibition of calcium entry and a reduction in the release of various neurotransmitters, including GABA, vasopressin, somatostatin, insulin and glucagons.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.