Thermo Fisher Scientific IL-6 Polyclonal Antibody
인간 IL-6 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ELISA 등 다양한 응용에 적합합니다. 단백질 A로 정제된 비결합 항체이며, IL-6의 중화 및 검출 연구에 활용됩니다. 장기 보관 시 -20°C 이하에서 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300–1:1,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with mature length recombinant human IL-6 produced in E. coli. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below. Avoid repeated freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid; dilute only before immediate use.
IL-6 antibody detects recombinant and native IL-6 present in body fluids and cell supernatants. In Western blot analysis, multiple IL-6 bands may appear due to variable glycosylation.
This antiserum is also useful for neutralization of human IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before testing.
A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended.
In vitro, this antibody does not enhance IL-6 activity. However, in vivo some antibodies to IL-6 may act as carriers and enhance IL-6 activity.
This product has minimal reactivity with mouse IL-6.
Target Information
IL-6 encodes a cytokine involved in inflammation and B cell maturation. The protein acts as an endogenous pyrogen that can induce fever in autoimmune diseases or infections.
It is primarily expressed at sites of acute and chronic inflammation, secreted into serum, and induces transcriptional inflammatory responses through the IL-6 receptor alpha.
IL-6 function is implicated in numerous inflammation-associated diseases, including diabetes mellitus and systemic juvenile rheumatoid arthritis.
Elevated IL-6 levels have also been observed in viral infections such as COVID-19 (caused by SARS-CoV-2).
Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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