Thermo Fisher Scientific TNF alpha Polyclonal Antibody
인간 TNF alpha에 특이적인 Rabbit Polyclonal Antibody로 Western blot, IHC, ELISA, IP 등 다양한 응용에 사용 가능. TNF alpha의 성숙형 및 전구체 단백질 검출에 적합하며, 프라이메이트 TNF alpha도 인식. -20°C에서 장기 보관 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| ELISA | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TNF alpha antibody produced by repeated immunizations with recombinant human TNF alpha (amino acids 1–233). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot and freeze at -20°C or below for long-term storage.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody is primarily directed against mature 17 kDa human TNF alpha and also detects primate TNF alpha in similar assays. It does not recognize human TNF beta (lymphotoxin). The antibody recognizes the 26 kDa cell-bound precursor of TNF alpha in immunoblots, especially under denaturing conditions. It is also effective for neutralizing human and primate TNF alpha activity in bioassays but does not neutralize lymphotoxin. For neutralization, incubate the sample with a 1:200 dilution of antibody for at least 4 hours before testing. A control using normal rabbit IgG at the same dilution is recommended.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. It is mainly secreted by macrophages and binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR receptors. TNF alpha regulates immune responses, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. It typically appears as a 17 kDa band on SDS-PAGE under non-reducing conditions. TNF alpha plays roles in immune defense against infections, tumor necrosis, and has been implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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