Thermo Fisher Scientific Phospho-Nemo-like kinase (Thr298) Polyclonal Antibody
Phospho-Nemo-like kinase (Thr298) 인산화 부위를 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. 고순도 Protein A 정제 제품으로 안정적인 신호 검출에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human Nemo-like kinase around the phosphorylation site of Thr298 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
NLK (Nemo-like kinase) is a serine/threonine kinase involved in signal transduction and cellular communication. It acts as a positive effector of the non-canonical Wnt signaling pathway, functioning downstream of WNT5A, MAP3K7/TAK1, and HIPK2. Activation leads to phosphorylation of the histone methyltransferase SETDB1, forming a complex that interacts with PPARG and results in methylation and transcriptional silencing of PPARG target genes. This process inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells. NLK also negatively regulates canonical Wnt/β-catenin, Notch, and MYB signaling pathways through phosphorylation-mediated inhibition of transcriptional complexes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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