Thermo Fisher Scientific Phospho-FAK (Tyr925) Polyclonal Antibody
FAK 단백질의 Tyr925 인산화 부위를 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF, Flow cytometry, ELISA 등 다양한 응용 가능. Human, Mouse, Rat 반응성. Protein A로 정제된 액상형 항체로 연구용에 적합.
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Thermo Fisher Scientific Phospho-FAK (Tyr925) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human FAK around the phosphorylation site of Tyr925 (Phospho-FAK Tyr925) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.03% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that serves as a substrate for Src and plays a key role in integrin signaling. It is involved in cell spreading, differentiation, migration, apoptosis, and the G1–S phase transition. FAK localizes to focal adhesions, which mediate cell attachment to the extracellular matrix via integrin receptors. Tyrosine phosphorylation of FAK increases upon integrin engagement with fibronectin. In murine NIH3T3 fibroblasts, adhesion to fibronectin promotes association of Grb2 and c-Src with FAK, activating ERK2 MAP kinase. Tyr925 has been identified as a critical phosphorylation site for Grb2 SH2 domain binding. Multiple transcript variants of FAK exist, with three full-length isoforms characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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