Thermo Fisher Scientific CD27 Monoclonal Antibody (O323), FITC, eBioscience
인간 CD27을 인식하는 O323 클론의 단일클론 항체로, FITC 형광 표지되어 흐름세포분석(Flow Cytometry)에 최적화됨. 인간 T세포 및 B세포 상호작용 연구에 적합하며, 488 nm 여기, 520 nm 방출 특성을 가짐. 4°C 보관, 냉동 금지.
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Thermo Fisher Scientific CD27 Monoclonal Antibody (O323), FITC, eBioscience™
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (1 µg)/test
- Publications: View 19 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | O323 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10669045 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 615, 737, 805
- Brilliant Violet 421, 480, 605, 711
- eFluor 450
- NovaFluor Blue 610-70S, 660-120S, 690, 725, 760, 800
- NovaFluor Red 700, 725, 755
- NovaFluor UV 765
- NovaFluor Violet 690
- NovaFluor Yellow 590, 610, 660, 810
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation available
Product Specific Information
The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells, and is upregulated upon T-cell stimulation. CD27 binds to CD70 and plays a key role in T cell–B cell interaction.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells
- Recommended Use: 5 µL (1 µg) per test (final volume 100 µL, cell number 10⁵–10⁸ cells/test)
- Excitation/Emission: 488 nm / 520 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD27 is a 50 kDa member of the TNF receptor superfamily (including CD40 and CD30) involved in cell proliferation and apoptosis regulation. Expression of CD27 and its ligand CD70 is mainly confined to lymphocytes. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells, and a subset of B cells.
Activation of T cells via TCR-CD3 complex upregulates CD27 expression and releases soluble CD27 (sCD27, 28–32 kDa), detectable in plasma, urine, or spinal fluid. Soluble CD27 serves as a prognostic marker for B cell malignancies. CD27-binding protein (SIVA) interacts with CD27 and contributes to apoptosis induction. Dysfunctions in CD27 are associated with Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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