Thermo Fisher Scientific CD27 Monoclonal Antibody (O323), FITC, eBioscience

Thermo Fisher Scientific CD27 Monoclonal Antibody (O323), FITC, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (1 µg)/test
• Publications: View 19 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 615, 737, 805
• Brilliant Violet 421, 480, 605, 711
• eFluor 450
• NovaFluor Blue 610-70S, 660-120S, 690, 725, 760, 800
• NovaFluor Red 700, 725, 755
• NovaFluor UV 765
• NovaFluor Violet 690
• NovaFluor Yellow 590, 610, 660, 810
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells, and is upregulated upon T-cell stimulation. CD27 binds to CD70 and plays a key role in T cell–B cell interaction.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells
• Recommended Use: 5 µL (1 µg) per test (final volume 100 µL, cell number 10⁵–10⁸ cells/test)
• Excitation/Emission: 488 nm / 520 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD27 is a 50 kDa member of the TNF receptor superfamily (including CD40 and CD30) involved in cell proliferation and apoptosis regulation. Expression of CD27 and its ligand CD70 is mainly confined to lymphocytes. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells, and a subset of B cells.

Activation of T cells via TCR-CD3 complex upregulates CD27 expression and releases soluble CD27 (sCD27, 28–32 kDa), detectable in plasma, urine, or spinal fluid. Soluble CD27 serves as a prognostic marker for B cell malignancies. CD27-binding protein (SIVA) interacts with CD27 and contributes to apoptosis induction. Dysfunctions in CD27 are associated with Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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