Thermo Fisher Scientific LC3A Polyclonal Antibody
LC3A 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS 버퍼에 0.02% sodium azide 포함. 단기 4°C, 장기 -20°C 보관 권장.
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Thermo Fisher Scientific LC3A Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,500 | View 2 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:400 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Flow Cytometry (Flow) | 1:100 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat, Zebrafish |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Genomic sequence corresponding to the N-terminal portion of the human LC3A protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154460 |
Product Specific Information
In ICC/IF, autophagosome formation has been observed in HeLa cells after treatment with 50 μM chloroquine.
Suggested positive control: human brain lysate, LC3 overexpression lysate.
Target Information
MAP1A and MAP1B are microtubule-associated proteins that mediate interactions between microtubules and cytoskeletal components.
The LC3A protein encoded by this gene is one of the light chain subunits that can associate with either MAP1A or MAP1B.
Two transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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