Thermo Fisher Scientific Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright 600, eBioscience
Flow cytometry용 Mouse IgG1 kappa 동형 대조 항체로, Super Bright 600 형광체 결합. Violet laser(405 nm)에서 여기되어 600 nm에서 방출. 실험 항체와 동일 농도로 사용하며, 비특이적 결합 최소화를 위해 Super Bright Staining Buffer 권장. 연구용 전용 제품.
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- 63-4714-8x (2개 옵션)
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Thermo Fisher Scientific Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright 600
Applications
- Flow Cytometry (Flow): Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | P3.6.2.8.1 |
| Conjugate | Super Bright™ 600 |
| Excitation/Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637447 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780, Biotin
- Brilliant UV series (395, 496, 563, 615, 661, 737, 805)
- Brilliant Violet series (421, 480, 605, 650, 711, 786)
- eFluor series (450, 506, 570, 615, 660)
- FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 645, 702, 780
- Request custom conjugation available
Product Specific Information
The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.
Applications Reported: Reported for use in flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes. Use at the same concentration as the experimental antibody.
Super Bright 600 is a tandem dye excited with the violet laser line (405 nm) and emits at 600 nm. Use a 610/20 bandpass filter. Ensure instrument compatibility.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 2–8°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Mouse IgG1 is a subtype of immunoglobulin G antibody produced by B cells in response to antigens. It is the most abundant IgG subtype in serum and plays a key role in the adaptive immune response. Composed of two heavy and two light chains, it participates in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. Widely used in research and diagnostic applications including ELISA, Western blotting, and immunohistochemistry, as well as in therapeutic research such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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