Thermo Fisher Scientific I-Ap Monoclonal Antibody (7-16.17), Biotin

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/1x10^6 cells

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

7-16.17

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• FITC
• PE
• Request custom conjugation

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 4-5mg/mL BSA

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539153

Product Specific Information

MA5-17768 targets I-Ap in FACS applications and shows reactivity with Mouse samples.

The MA5-17768 immunogen is b10.p.

Target Information

MHC Class II (I-Ap) is a protein complex found on the surface of cells in vertebrates. Its main function is to present peptides derived from extracellular proteins to T cells for immune recognition. MHC Class II (I-Ap) is composed of an alpha and beta chain, both of which contribute to the peptide-binding groove. MHC Class II (I-Ap) is primarily restricted to antigen-presenting cells, including dendritic cells, macrophages, and B cells. These cells are responsible for capturing and processing antigens from the extracellular environment. Once the antigens are internalized, they are broken down into smaller peptides, which then bind to MHC Class II molecules within the endosomal compartments of these cells. MHC Class II (I-Ap) consists of an extracellular domain, a transmembrane domain, and a cytoplasmic tail. The extracellular domain is responsible for interacting with the antigenic peptides, while the transmembrane domain anchors the MHC Class II molecule to the cell membrane. The cytoplasmic tail plays a role in intracellular signaling events.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.