Thermo Fisher Scientific I-Ek Monoclonal Antibody (14-4-4S), FITC

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

14-4-4S

Immunogen

C3H if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MHC Class II (I-Ek),uniProtId:P04230-1,ncbiNodeId:10090,antigenRange:1-264,antigenLength:264,antigenImageFileName:MA5-17762_MHC_Class_II_I-Ek_P04230-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17762_MHC_Class_II_I-Ek_P04230-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:MHC Class II (I-Ek),uniProtId:P14439-1,ncbiNodeId:10090,antigenRange:1-255,antigenLength:255,antigenImageFileName:MA5-17762_MHC_Class_II_I-Ek_P14439-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17762_MHC_Class_II_I-Ek_P14439-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

FITC FITC FITC

View additional formats

• Unconjugated
• Biotin
• PE
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 4-5mg/mL BSA

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539150

Product Specific Information

MA5-17762 targets I-Ek in FACS applications and shows reactivity with Mouse samples.

The MA5-17762 immunogen is c3H.

Target Information

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.