Thermo Fisher Scientific IL-12 Polyclonal Antibody
IL-12 폴리클로날 항체로 Rat 시료에 반응하며, WB, IHC, ICC, ELISA 등에 사용 가능. Rabbit IgG 기반으로 Protein A 정제. KLH 결합 합성 펩타이드(aa 141-200) 면역원 사용. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from rat IL-12 (amino acids 141–200) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Interleukin 12A (IL-12A) is a signaling cytokine belonging to the Interleukin-6 superfamily. It encodes the 35 kDa alpha subunit of IL-12, a heterodimeric cytokine with diverse biological functions. IL-12A acts as a growth factor for activated T and NK cells, enhancing NK and lymphokine-activated killer cell activity. It stimulates IFN-γ production in PBMCs and contributes to both Th1 and Th2 cell differentiation. IL-12A also positively regulates tyrosine phosphorylation of Stat4 protein and requires nitric oxide synthase 2A for innate immune signaling. Together with EBI3, IL-12A forms interleukin-35 (IL-35), produced by regulatory T cells and essential for suppressive activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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