Thermo Fisher Scientific SCIMP Polyclonal Antibody
SCIMP 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot 및 IHC(P)에서 검증됨. 항원 친화 크로마토그래피로 정제되었으며, PBS buffer(40% glycerol) 보존. Human 시료에 반응하며 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human SCIMP. Recombinant protein control fragment (Product # RP-88833) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2647004 |
Product Specific Information
Immunogen sequence:
RRGKKWEIAK PLKHKQVDEE KMYENVLNES PVQLPPLPPR NWPSLEDSSP QEAPSQPPAT YSLVNKVKNK KTVSIPSYIE PEDDYDDVEI PANTE
Highest antigen sequence identity to the following orthologs:
- Mouse: 65%
- Rat: 58%
Target Information
SCIMP (SLP adaptor and Csk interacting membrane protein), also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen-presenting cells, most prominently in the lymph nodes and spleen. It is associated with tetraspanin-enriched microdomains (together with MHC II).
There is a close relationship between SCIMP and tyrosine kinase Lyn, which is constitutively bound to it by its SH3 domain. After MHC II-mediated stimulation in the immunological synapse, SCIMP becomes phosphorylated at several tyrosine residues and provides docking sites for Grb2 and SLP65 or SLP76 adaptors, transducing the signal downstream, as well as for the kinase Csk with modulatory roles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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