Thermo Fisher Scientific CENPE Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence. Antigen range: 392-509. Recombinant protein control fragment (Product #RP-89483). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CENPE,uniProtId:Q02224-1,ncbiNodeId:9606,antigenRange:392-509,antigenLength:2701,antigenImageFileName:PA5-144699_CENPE_Q02224-1_Rabbit.svg,antigenImageFileNamePDP:PA5-144699_CENPE_Q02224-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3091885

Product Specific Information

Immunogen sequence: EKIENLTRML VTSSSLTLQQ ELKAKRKRRV TWCLGKINKM KNSNYADQFN IPTNITTKTH KLSINLLREI DESVCSESDV FSNTLDTLSE IEWNPATKLL NQENIESELN SLRADYDN

Antigen sequence identity: Mouse 56%; Rat 60%

Target Information

14-3-3 sigma (stratifin, SFN, epithelial cell marker protein 1) is a member of a highly conserved family of 14-3-3 proteins that are present in all eukaryotic organisms. There are 7 known mammalian 14-3-3 isoforms epsilon, beta, zeta, gamma, theta, tau and they play important roles in many biological activities by binding to and altering the subcellular localization and/or stability of key molecules in various signaling cascades. 14-3-3 sigma was originally characterized as a human mammary epithelium marker and later rediscovered as an important molecule for cell cycle checkpoint regulation. Recently it has been reported that 14-3-3 sigma may serve as a prognosis marker predicting survival of pancreatic cancer patient treatment. 14-3-3 sigmais the only 14-3-3 isoform induced by the tumor suppressor protein p53, in response to g-irradiation and other DNA-damaging agents. 14-3-3 sigmais a p53-regulated inhibitor of G2/M progression and acts as a tumor suppressor gene that is inactivated by methylation of its 5` CpG islands in epithelial tumor cells. Two isoforms of human 14-3-3 sigma are produced by alternative splicing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.