Thermo Fisher Scientific Ub-K48 Recombinant Rabbit Monoclonal Antibody (ARC0811)
Ub-K48 단백질을 인식하는 재조합 토끼 단클론 항체. Western blot, ICC/IF, ELISA에 적합. 인간, 마우스, 랫트 반응성. HEK293 세포 발현, Affinity chromatography로 정제. -20°C 보관, 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0811 |
| Immunogen | A synthetic peptide corresponding to amino acids 1–100 of human Ubiquitin (P0CG47) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849283 |
Product Specific Information
Immunogen sequence:
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGMQIF VKTLTGKTIT LEVEPSDTIE
Target Information
Ubiquitin exists either covalently attached to another protein or free (unanchored). When covalently bound, it forms monoubiquitin or polyubiquitin chains linked via different Lys residues or the initiator Met.
- Lys-6-linked: DNA repair
- Lys-11-linked: ERAD and cell-cycle regulation
- Lys-29-linked: Lysosomal degradation
- Lys-33-linked: Kinase modification
- Lys-48-linked: Proteasomal degradation
- Lys-63-linked: Endocytosis, DNA-damage response, NF-κB signaling
Linear polyubiquitin chains (Met-linked) are involved in cell signaling.
Free polyubiquitin also plays roles in kinase activation and signaling.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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