Thermo Fisher Scientific Human STK4 (MST1), GST Tag Recombinant Protein

Applications

• Kinase Assay (KA)

Tested Dilution: Assay-dependent
Publications: -

Product Specifications

Product Specific Information

• For maximum recovery, please spin prior to use.
• Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use material in original packaging until exhausted.
• For larger sizes, aliquoting can reduce freeze/thaw cycles; aliquots below 20 µL are not recommended.
• Never store kinase diluted.
• Properly stored at -80°C, product is guaranteed for 6 months from date of purchase.
• Protein Form: Recombinant, Full Length

Target Information

STK4 (MST1) is a serine/threonine kinase involved in apoptosis. It may induce chromatin condensation observed during this process. STK4 is a stress-activated, pro-apoptotic kinase that, after caspase cleavage, enters the nucleus and triggers chromatin condensation followed by DNA fragmentation.

It is a key component of the Hippo signaling pathway, crucial for organ size control and tumor suppression by limiting proliferation and promoting apoptosis. Within this cascade, STK3/MST2 and STK4/MST1 complexed with SAV1 phosphorylate and activate LATS1/2 (with MOB1), which then phosphorylate and inactivate YAP1 and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 prevents its nuclear translocation, regulating genes responsible for proliferation, cell death, and migration.

STK3/MST2 and STK4/MST1 are essential for repressing proliferation of mature hepatocytes, preventing activation of adult liver stem cells (oval cells), and inhibiting tumor formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.