Thermo Fisher Scientific RUNX2 Monoclonal Antibody (6E1)
RUNX2 단백질을 표적하는 Mouse IgG2b 단클론 항체로, Western blot, IHC, ELISA에 적합. 인간 시료 반응성, 액상 형태로 제공되며 PBS 버퍼에 보존제 없음. 골형성 및 골분화 연구에 유용.
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Thermo Fisher Scientific RUNX2 Monoclonal Antibody (6E1)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 3 µg/mL |
| ELISA | 0.3 ng/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6E1 |
| Immunogen | RUNX2 (NP_004339, 251–350 a.a.) partial recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
NPRPSLNSAP SPFNPQGQSQ ITDPRQAQSS PPWSYDQSYP SYLSQMTSPS IHSTTPLSST RGTGLPAITD VPRRISDDDT ATSDFCLWPS TLSKKSQAGA
Target Information
RUNX2 is a nuclear protein and a member of the RUNX family of transcription factors with a Runt DNA-binding domain. It is essential for membranous and endochondral bone formation, regulating osteoblastic differentiation and skeletal morphogenesis. RUNX2 acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression, binds DNA as a monomer or heterodimer, and increases TGFBR1 expression by osteoblasts. It cooperates with DLX5 or related factors to activate osteoblast-specific genes. Mutations in RUNX2 can lead to bone development disorders such as cleidocranial dysplasia (CCD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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