Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), NovaFluor Blue 760, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), NovaFluor Blue 760, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 0.1 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 647, 660, 700
• APC, APC-eFluor 780, Biotin
• Brilliant UV 395–805 series
• Brilliant Violet 421–786 series
• eFluor 450, 506, 570, 615
• FITC, PE, PE-Cyanine5/5.5/7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780 series
• NovaFluor Blue and Yellow series
• Pacific Orange
• Custom conjugation available

Product Specific Information

The RM4-5 monoclonal antibody reacts with mouse CD4, a 55 kDa cell surface receptor expressed by thymocytes, mature T cells, and dendritic cells. CD4 binds MHC class II molecules and plays a key role in T cell development and function through association with p56lck kinase. Binding of RM4-5 is blocked by GK1.5.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse thymocytes and splenocytes (≤0.1 µg/test; 100 µL final volume; 10⁵–10⁸ cells/test).
• Optimization: Carefully titrate for best performance.

NovaFluor dyes compatibility:
Not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

Buffer recommendation:
Shipped with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01). Add 5 µL per sample to antibody cocktails or single-color controls for optimal staining.

Technology:
NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for minimal spectral spillover.

• Excitation: 492 nm
• Emission: 766 nm
• Laser: 488 nm (Blue)

Target Information

CD4 antigen functions in recognition of MHC class II molecules and serves as a co-receptor for HIV. Expressed mainly in T helper cells, monocytes, macrophages, and dendritic cells. Found in thymus, lymph nodes, tonsils, spleen, and various non-lymphoid tissues. CD4 aids T-cell activation and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts exist (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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