Thermo Fisher Scientific WASP Monoclonal Antibody (7B10E4)
WASP 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, Flow cytometry, ELISA 등에 사용 가능. Human 시료에 반응하며, Protein G로 정제된 액상 형태. 단기 4°C, 장기 -20°C 보관 권장. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7B10E4 |
| Immunogen | Purified recombinant fragment of human WAS (AA: 57–170) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787575 |
Product Specific Information
MA5-31952 has been tested in indirect ELISA.
Target Information
The Wiskott-Aldrich syndrome family of proteins share similar domain structures and are involved in signal transduction from cell surface receptors to the actin cytoskeleton. They are regulated by multiple stimuli and interact with various proteins. These proteins associate with the small GTPase Cdc42, which regulates actin filament formation, and the Arp2/3 complex.
Wiskott-Aldrich syndrome is a rare, inherited, X-linked recessive disease characterized by immune dysregulation and microthrombocytopenia, caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein expressed exclusively in hematopoietic cells, which show signaling and cytoskeletal abnormalities in patients. A transcript variant with a different 5′ UTR sequence has been described, though its full-length nature remains unknown.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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