Thermo Fisher Scientific E2F1 Recombinant Rabbit Monoclonal Antibody (1D12)
E2F1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등에 사용 가능. 높은 특이성과 재현성을 제공하며, PBS/glycerol 버퍼에 보존. 세포주기 및 종양억제 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:20–1:200 |
| Flow Cytometry (Flow) | 1:20–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 1D12 |
| Immunogen | A synthesized peptide derived from human E2F1 (UniProt ID: Q01094-1, range: 381–394, length: 437 aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.35 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 150 mM NaCl |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2898294 |
Product Specific Information
This antibody has been tested in direct ELISA.
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of the cell cycle and the action of tumor suppressor proteins. It is also a target of transforming proteins of small DNA tumor viruses. E2F proteins contain several conserved domains, including a DNA-binding domain, a dimerization domain for interaction with DP proteins, a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain embedded within the transactivation domain. E2F1, along with E2F2 and E2F3, has an additional cyclin-binding domain and binds preferentially to retinoblastoma protein (pRB) in a cell-cycle-dependent manner, mediating both cell proliferation and p53-dependent/independent apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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