Thermo Fisher Scientific ATP5J Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:15-1:50

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full length fusion protein of Human ATP5J if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ATP5J,uniProtId:P18859-1,ncbiNodeId:9606,antigenRange:1-108,antigenLength:108,antigenImageFileName:PA5-50201_ATP5J_P18859-1_Rabbit.svg,antigenImageFileNamePDP:PA5-50201_ATP5J_P18859-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.6 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 40% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2635654

Product Specific Information

The antibody detects endogenous levels of total ATP5J protein.

Target Information

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The F0 seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the F0 complex, required for F1 and F0 interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.