Thermo Fisher Scientific HSD3B2 Polyclonal Antibody
HSD3B2 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF 등 다양한 응용에 적합. 인간 및 생쥐 반응성 확인. 고순도 항원 친화 크로마토그래피 정제. 장기 보관 시 -20°C 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 3 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 126–372 of Human HSD3B2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.24 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545267 |
Product Specific Information
- Recommended positive controls: HeLa, Raji
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
3 beta-hydroxysteroid dehydrogenase (3β-HSD), also known as HSD3B1 or HSDB3, is a bifunctional enzyme crucial in the synthesis of all classes of hormonal steroids.
Two human 3β-HSD proteins, type I (3β-HSD) and type II (3β-HSD2), are encoded by different genes and function in distinct tissues.
Localized to the endoplasmic reticulum membrane and expressed in skin and placenta, type I 3β-HSD catalyzes the oxidative conversion of Δ5-ene-3β-hydroxy steroids and various ketosteroids.
Defects in 3β-HSD genes are associated with salt wasting, genital ambiguity, hypogonadism, insulin-resistant PCOS, and increased prostate cancer susceptibility.
Congenital deficiency of 3β-HSD activity leads to severe steroid formation depletion, which can be lethal in young children.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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