Thermo Fisher Scientific NEK9 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

-

Product Specifications

Species Reactivity

Horse, Hamster, Human, Non-human primate, Pig, Rabbit

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic 15 amino acid peptide from Pro/Ser/Thr rich domain of human NEK9.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2551329

Product Specific Information

Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Elephant, Panda, Horse, Rabbit, Pig (100%); Mouse, Rat, Dog, Bovine, Bat, Opossum (93%); Platypus (87%); Turkey, Chicken (80%).

Proteinase K treatment is required for antigen retrieval.

Target Information

NEK9, a NEK type protein kinase, regulates chromosome alignment and segregation in mitosis. The protein has a N-terminal NIMA-like catalytic domain, a central domain with homology to the guanine nucleotide exchange factor for the GTPase Ran (RCC1), and a C-terminal coiled-coil domain. It is phosphorylated by active p34(Cdc2) and is capable of autophosphorylation and oligomerization. The activated protein binds Nek6 and RCC1, and has been shown to associate with the human homolog of the Drosophila protein Bicaudal D (Bicd2) in vivo and to phosphorylate this coiled-coil protein in vitro. NEK9 expression has been documented in normal human brain. ESTs have been isolated from several tissue libraries, including normal bone, lymph and thymus and cancerous cervix, eye, lymph and placenta.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.