Thermo Fisher Scientific IFIT3 Polyclonal Antibody
Human IFIT3 단백질에 특이적인 Rabbit Polyclonal Antibody로, WB, IHC(P), ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS buffer(40% glycerol)로 보관. 항바이러스 반응 및 세포 신호 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human IFIT3. Recombinant protein control fragment (Product # RP-101235) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2791589 |
Product Specific Information
Immunogen sequence:
HKQNGDLLQA AKCYEKELGR LLRDAPSGIG SIFLSASELE DGSEEMGQGA VSSSPRELLS NSEQL
Target Information
IFN-induced antiviral protein which acts as an inhibitor of cellular and viral processes, including cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by bridging TBK1 to MAVS, leading to TBK1 activation and IRF3 phosphorylation. Phosphorylated IRF3 translocates into the nucleus to promote antiviral gene transcription.
Exhibits antiproliferative activity via upregulation of CDKN1A/p21 and CDKN1B/p27. IFIT3 sequesters COPS5 in the cytoplasm, increasing nuclear CDKN1B/p27 levels and upregulating CDKN1A/p21 by downregulating MYC. Can negatively regulate the apoptotic effects of IFIT2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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