Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES5-2A5), Biotin, eBioscience
Mouse IL-10을 인식하는 중화용 단클론 항체로, Biotin으로 표지되어 ELISA 및 Flow Cytometry에 최적화됨. JES5-2A5 클론으로 높은 특이성과 재현성을 제공. 연구용으로 면역조절 및 염증 반응 분석에 적합.
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Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES5-2A5), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry | - | View 1 publication |
| ELISA | 0.5–2 µg/mL | View 6 publications |
| Neutralization | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | JES5-2A5 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466918 |
| Filtration | 0.2 µm post-manufacturing filtered |
Related Formats
Product Specific Information
The JES5-2A5 antibody reacts with mouse interleukin-10 (IL-10) and functions as a neutralizing antibody. Mouse IL-10 (18 kDa) is also known as Cytokine Synthesis Inhibitory Factor (CSIF). In mice, Th2 cells, B1 cells, macrophages, and keratinocytes produce IL-10. IL-10 inhibits Th1 cytokine synthesis and T cell proliferation, while acting as a costimulatory signal for mast cells, developing thymocytes, and the Th2 response.
Applications Reported: ELISA, ELISPOT, neutralization of IL-10 bioactivity, and intracellular staining for flow cytometric analysis.
Applications Tested: Detection antibody in sandwich ELISA for mouse IL-10, used with JES5-16E3 (14-7101) for capture and recombinant mouse IL-10 (14-8101) as standard. Recommended range: 0.5–2.0 µg/mL. Standard curve: 4000 pg/mL–30 pg/mL.
Target Information
IL-10 encodes a cytokine mainly produced by monocytes and some lymphocytes. It regulates immune responses and inflammation by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. IL-10 enhances B cell survival, proliferation, and antibody production, blocks NF-kappa B activity, and participates in JAK-STAT pathway regulation.
Knockout studies show IL-10 is essential for intestinal immune regulation. Mutations in IL10 are linked to increased susceptibility to HIV-1 infection and rheumatoid arthritis. Associated diseases include Graft-Versus-Host Disease and HIV Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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