Thermo Fisher Scientific PUS10 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human PUS10. Recombinant protein control fragment (Product #RP-99688). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PUS10,uniProtId:Q3MIT2-1,ncbiNodeId:9606,antigenRange:270-375,antigenLength:529,antigenImageFileName:PA5-62015_PUS10_Q3MIT2-1_Rabbit.svg,antigenImageFileNamePDP:PA5-62015_PUS10_Q3MIT2-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2646150

Product Specific Information

Immunogen sequence: NSPKAVCAVL EIECAHGAVF VAGRYNKYSR NLPQTPWIID GERKLESSVE ELISDHLLAV FKAESFNFSS SGREDVDVRT LGNGRPFAIE LVNPHRVHFT SQEIKE

Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 92%.

Target Information

Pseudouridylate synthases catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. PUS10 catalyzes the formation of the universal psi55 in the GC loop of transfer RNAs (Probable). Modulator of TRAIL-induced cell death via activation of procaspase 8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.