Thermo Fisher Scientific F(ab`)2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, Qdot 625

Applications

Tested Dilution

Western Blot (WB)

1:50

Immunohistochemistry (IHC)

1:50

Immunocytochemistry (ICC/IF)

4 µg/mL

Flow Cytometry (Flow)

1:50

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Qdot™ 625 Qdot™ 625 Qdot™ 625

View additional formats

• [Alexa Fluor 488-10 nm colloidal gold](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Alexa Fluor 488-10 nm colloidal gold/host/Goat/)
• [Alexa Fluor 488-5 nm colloidal gold](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Alexa Fluor 488-5 nm colloidal gold/host/Goat/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Alexa Fluor 568/host/Goat/)
• [AP](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/AP/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Biotin/host/Goat/)
• [DyLight 350](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 350/host/Goat/)
• [DyLight 405](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 405/host/Goat/)
• [DyLight 488](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 488/host/Goat/)
• [DyLight 550](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 550/host/Goat/)
• [DyLight 594](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 594/host/Goat/)
• [DyLight 633](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 633/host/Goat/)
• [DyLight 650](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 650/host/Goat/)
• [DyLight 680](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 680/host/Goat/)
• [DyLight 800 4X PEG](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 800 4X PEG/host/Goat/)
• [FITC](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/FITC/host/Goat/)
• [HRP](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/HRP/host/Goat/)
• [NANOGOLD](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/NANOGOLD/host/Goat/)
• [PE ](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/PE/host/Goat/)
• [PE-Cyanine5.5](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/PE-Cyanine5.5/host/Goat/)
• [Qdot 525](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 525/host/Goat/)
• [Qdot 585](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 585/host/Goat/)
• [Qdot 605](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 605/host/Goat/)
• [Qdot 655](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 655/host/Goat/)
• [Qdot 705](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 705/host/Goat/)
• [Qdot 800](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 800/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/TRITC/host/Goat/)
• [WesternDot 625](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/WesternDot 625/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

300/621 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

0.05M borate, pH 8.3, with 1M betaine

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient

RRID

AB_2534019

Target

IgG

Antibody Form

F(ab`)2 Fragment

Product Specific Information

Qdot nanocrystals are composed of semi-conductor material to generate a fluorescent particle which is exceptionally bright and does not photobleach. Qdot nanocrystals paired with the correct optical filters are as much as 50 times brighter than traditional organic dyes.

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.