Thermo Fisher Scientific Qtracker 625 Cell Labeling Kit

Invitrogen™ Qtracker™ 625 Cell Labeling Kit

The Qtracker™ 625 Cell Labeling Kit uses a custom targeting peptide to deliver orange/red-fluorescent Qdot™ 625 nanocrystals into the cytoplasm of live cells.
Qtracker™ kits are designed to load cultured cells with highly fluorescent Qdot™ nanocrystals, enabling long-term tracking without transfer to adjacent cells.

Need a different emission spectrum or longer tracking? View other mammalian cell tracking products.

Key Attributes

• Qtracker™ 625 label has Ex/Em (405–585/625) nm
• Designed for loading cultured cells with Qdot™ nanocrystals
• Provides intense, stable fluorescence traceable through several generations
• Available in eight colors: 525, 565, 585, 605, 625, 655, 705, 800 nm emission
• Ideal for long-term tracking or imaging of live cells (migration, motility, morphology, and functional assays)

Long-Lasting, Targeted Signal

Qtracker™ labels enable observation under continuous illumination without photobleaching or degradation.
The labels are distributed in cytoplasmic vesicles and inherited by daughter cells for at least six generations.
Fluorescence can persist up to a week post-labeling, making it ideal for long-term studies.

Monitor the Signal on Multiple Platforms

Labeled live cells can be monitored using:

• Flow cytometry
• Fluorescence/confocal microscopy
• Fluorescence microplate readers
• High-content imaging systems

Minimal Impact on Live Cells

Tested on multiple cell lines (CHO, HeLa, U-118, 3T3, HUVEC, Jurkat), Qtracker™ labeling shows minimal impact on:

• Surface marker expression
• Cell proliferation
• Enzyme activity
• Cell motility

Applications

Qtracker™ labeled cells are useful for:

• Co-culture and heterotypic assembly
• Multilineage differentiation
• Trans-differentiation vs. cell fusion studies
• Embryonic and mesenchymal stem cell tracking
• Cell migration dynamics

Specifications

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