Thermo Fisher Scientific DDHD2 Polyclonal Antibody
Human DDHD2 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC(P), ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS buffer(40% glycerol)에서 보관. 세포 내 인지질 가수분해 효소 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human DDHD2 (Product #RP-93744) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Wet ice |
| RRID | AB_2640446 |
Product Specific Information
Immunogen sequence:
RMHLELREGL TRMSMDLKNN LLGSLRMAWK SFTRAPYPAL QASETPEETE AEPESTSEKP SDVNTEETSV AVKEEVLPIN VGMLNGGQRI DYVLQEK
Highest antigen sequence identity to the following orthologs:
- Mouse: 87%
- Rat: 88%
Target Information
DDHD2 (also known as KIAA0725p) is a member of the intracellular phospholipase A1 (PLA1) protein family. These enzymes hydrolyze the sn-1 ester bond of phospholipids, producing 2-acyl-lysophospholipids and fatty acids.
DDHD2 prefers phosphatidic acid as a substrate and plays a role in efficient membrane trafficking from the Golgi apparatus to the plasma membrane.
The DDHD2 gene is located on chromosome 8p11.23 and encodes a 711-amino-acid protein with a calculated molecular mass of 81 kDa.
Immunoblot analysis of various tissue extracts revealed two bands of approximately 90 and 85 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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