Thermo Fisher Scientific ATF3 Polyclonal Antibody
ATF3 단백질을 검출하기 위한 Rabbit Polyclonal 항체로, Western blot, IHC(P), IP에 사용 가능. Human 및 Rat에 반응하며, 항원 친화 크로마토그래피로 정제됨. PBS/glycerol 완충액에 보관되며 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | – |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | – |
| Immunoprecipitation (IP) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 120–170 of human ATF3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553436 |
Product Specific Information
- Detects endogenous ATF3 protein at approximately 21 kDa.
- Purity >95% by SDS-PAGE.
Target Information
ATF3 (Activating Transcription Factor 3) is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family. It encodes a protein with a molecular mass of approximately 22 kDa and generally represses transcription from promoters with ATF binding elements.
An alternatively spliced variant (ATF3 delta Zip) lacks the leucine zipper domain, does not bind DNA, and can stimulate transcription by sequestering inhibitory cofactors. Alternative splicing of the ATF3 gene may play an important role in gene regulation. Three transcript variants encoding two isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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