Thermo Fisher Scientific F(ab`)2-Rabbit anti-Goat IgG (H+L) Secondary Antibody, Biotin
Rabbit에서 생산된 F(ab')2 형태의 anti-Goat IgG (H+L) 2차 항체로 Biotin 결합형입니다. Western blot, ICC/IF, Flow cytometry, ELISA 등 다양한 응용에 적합합니다. 고순도 Affinity Chromatography 정제 및 높은 특이성과 감도를 제공합니다.
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Thermo Fisher Scientific F(ab`)2-Rabbit anti-Goat IgG (H+L) Secondary Antibody, Biotin
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535919 |
| Target | IgG |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
Each lot of antibody is tested for sensitivity using ELISA and for specificity using immunoelectrophoresis (IEP).
Target Information
Anti-Goat secondary antibodies are affinity-purified with well-characterized specificity for goat immunoglobulins. They are useful for detection, sorting, or purification of goat IgG targets.
Secondary antibodies provide versatility and enhanced sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, these antibodies are produced by immunizing the host with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Formats
- AP conjugate
- FITC conjugate
- HRP conjugate
- Qdot 655 conjugate
- TRITC conjugate
- Custom conjugation available upon request
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