Thermo Fisher Scientific F(ab`)2-Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
HRP 결합 F(ab')2 형태의 Rabbit anti-Goat IgG (H+L) 2차 항체로 Western blot, IHC, ELISA에 적합. 인간, 마우스, 랫 IgG에 대해 교차흡착 처리되어 높은 특이성과 신뢰성 제공. 친화 크로마토그래피로 정제된 동결건조 제품.
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Thermo Fisher Scientific F(ab`)2-Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535921 |
| Target | IgG |
| Cross Adsorption | Against human, mouse and rat IgG |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.)
Centrifuge to remove particulates. Prepare fresh working dilution daily.
Store lyophilized material at 2–8°C.
For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin goat serum immunoglobulins, or IgG from human, mouse, or rat.
Target Information
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins.
They are useful in detection, sorting, or purification of the specified target.
Secondary antibodies offer versatility for various detection systems (HRP, AP, fluorescence) and provide greater sensitivity through signal amplification.
Typically generated by immunizing the host animal with pooled immunoglobulins from the target species, then purified and modified (immunoaffinity chromatography, fragmentation, label conjugation) to yield highly specific reagents.
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 633
- Alexa Fluor 647
- Alexa Fluor 680
- AP
- Biotin
- Biotin-XX
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 800
- FITC
- TRITC
- Request custom conjugation available
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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