Thermo Fisher Scientific AP2M1 Polyclonal Antibody
AP2M1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot 및 IHC(P) 검증됨. 다양한 종에 예측 반응성 있음. 항원 친화 크로마토그래피로 정제된 액상 항체로 연구용에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to amino acids 1–150 (Q96CW1) of AP2M1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.57 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11154082 |
Product Specific Information
- Recommended positive controls: 293T, A431, H1299, HeLaS3, Raji
- Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (99%), Dog (100%), Pig (99%), Chicken (97%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase responsible for proton pumping during endosome and lysosome acidification.
AP2M1 associates with the HIV-1 protein Nef, suggesting Nef may use the AP-2 complex to enhance endocytosis of CD4 and class I MHC. AP2M1 may also play a role in regulating intracellular trafficking and function of CTLA-4 protein. At least two isoforms of AP2M1 are known to exist.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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