Thermo Fisher Scientific Agrin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 30-350 of human AGRN (NP_940978.2). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Agrin,uniProtId:O00468-1,ncbiNodeId:9606,antigenRange:30-350,antigenLength:2068,antigenImageFileName:PA5-109369_Agrin_O00468-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109369_Agrin_O00468-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.67 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2854780

Product Specific Information

Immunogen sequence: TCPERALERR EEEANVVLTG TVEEILNVDP VQHTYSCKVR VWRYLKGKDL VARESLLDGG NKVVISGFGD PLICDNQVST GDTRIFFVNP APPYLWPAHK NELMLNSSLM RITLRNLEEV EFCVEDKPGT HFTPVPPTPP DACRGMLCGF GAVCEPNAEG PGRASCVCKK SPCPSVVAPV CGSDASTYSN ECELQRAQCS QQRRIRLLSR GPCGSRDPCS NVTCSFGSTC ARSADGLTAS CLCPATCRGA PEGTVCGSDG ADYPGECQLL RRACARQENV FKKFDGPCDP CQGALPDPSR SCRVNPRTRR PEMLLRPESC P

Target Information

Agrin is a molecule that resides in the basal lamina of muscle cells and directs key events in post synaptic differentiation. Most notably, Agrin is responsible for the clustering of acetylcholine receptors (AChRs) on the cell surface and their localization to the neuromuscular junction. Several Agrin variants have been identified which arise from alternative mRNA splicings. Agrin splice forms having inserts at two sites in the carboxy terminus designated y and z display a high affinity for AChRs, while splice forms lacking these inserts associate with AChRs weakly. Muscle alpha-dystroglycan has been postulated to be the receptor for the clustering activity of agrin; however, this is a point of contention. Tyrosine phosphorylation has been implicated as a required early step in AChR aggregation. Interestingly, a unique receptor tyrosine kinase, designated MuSK, has been discovered that interacts with Agrin and is specifically localized to developing muscle.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.