Thermo Fisher Scientific Cytokeratin 18 Polyclonal Antibody
Human Cytokeratin 18을 인식하는 Rabbit Polyclonal Antibody로 WB, IHC, ICC, Flow, IP 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태로 제공. 조직 염색 전 열매개 항원 회수 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
| Flow Cytometry (Flow) | 1 µg for 10⁶ cells |
| Immunoprecipitation (IP) | 5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human Cytokeratin 18 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10983001 |
Product Specific Information
- Heat-mediated antigen retrieval is recommended prior to tissue staining.
- This antibody is predicted to react with mouse and rat based on sequence homology.
Target Information
Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with cytokeratin 8.
It is expressed in tissues from the gastrointestinal, respiratory, and urogenital tracts, as well as in endocrine, exocrine, and mesothelial cells.
Cytokeratin 18 is mainly found in non-squamous epithelia and is present in most adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas.
Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis.
Two transcript variants encoding the same protein have been identified for the cytokeratin 18 gene.
Cytokeratin subtype expression patterns are increasingly used to distinguish different epithelial malignancies.
Cytokeratin antibodies are useful in tumor differential diagnosis using immunohistochemistry, cytopathology, and flow cytometry assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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