Thermo Fisher Scientific TPPP Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.001-0.01 µg/mL

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Immunohistochemistry (IHC)

-

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Immunohistochemistry (Paraffin) (IHC (P))

3.75 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

-

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Immunoprecipitation (IP)

-

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (DPSKDRAAKRLSLES) corresponding to the internal amino acids of TPPP (aa 21-35). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TPPP,uniProtId:O94811-1,ncbiNodeId:9606,antigenRange:21-35,antigenLength:219,antigenImageFileName:PA5-19243_TPPP_O94811-1_Goat.svg,antigenImageFileNamePDP:PA5-19243_TPPP_O94811-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10979400

Product Specific Information

This antibody is predicted to react with porcine based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.

Target Information

Tubulin family members are globular proteins important in the assembly of microtubules. Microtubules are structural components that play important roles in mitosis, cytokinesis and vesicle transport. TPPP (Tubulin polymerization-promoting protein), also known as p24 and p25, is a widely expressed 219 amino acid protein found in the perinuclear region of the cytoplasm. TPPP may form dimers and functions in polymerizing tubulin into double-walled tubules, polymorphic aggregates, or stabilized blocks. TPPP overexpression prevents formation of the mitotic spindle assembly and breakdown of the nuclear envelope. TPPP is phosphorylated by TPK II and is encoded by a gene that maps to human chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome. May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.