Thermo Fisher Scientific eIF4G3 Polyclonal Antibody
Human, Mouse, Rat에서 반응하는 eIF4G3 폴리클로날 항체로, WB, IHC, ICC, IP 등 다양한 응용에 적합. 고순도 항원 친화 크로마토그래피 정제. 안정적 액상 형태로 장기 보관 시 -20°C 권장.
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Thermo Fisher Scientific eIF4G3 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:20,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to a region within amino acids 1–203 of Human EIF4G3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.26 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2548575 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse testis
- Predicted reactivity: Human (99%), Mouse (96%), Rat (97%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is part of the eIF4F complex, involved in mRNA cap recognition and transport to the ribosome.
MicroRNA miR-520c-3p negatively regulates this protein, leading to reduced protein translation and cell proliferation.
Thus, eIF4G3 is a key component of the anti-tumor activity of miR-520c-3p.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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