Thermo Fisher Scientific VIM Monoclonal Antibody (1G3)
사람 Vimentin 단백질을 인식하는 마우스 단클론 항체입니다. Western blot과 ELISA에 적합하며, 비결합형 액상 형태로 제공됩니다. PBS(pH 7.4) 버퍼에 보관하며, -20°C에서 냉동 보관이 권장됩니다. 연구용으로만 사용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1–5 µg/mL
ELISA
- Tested Dilution: 10 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1G3 |
| Immunogen | A synthetic peptide corresponding to human VIM (amino acids 366–390) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
QDEIQNMKEE MARHLREYQD LLNVK
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFPs, vimentin is expressed along with desmin during early cellular development and later replaced by tissue-specific IFPs.
Vimentin also plays a role in steroid synthesis by serving as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase leads to filament breakdown and cytoskeletal reorganization, facilitating cholesterol transport to mitochondria and enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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