Takara VpaK11BI (AvaII) restriction enzyme

VpaK11BI (AvaII) restriction site:

G | G(A/T)C C
C C(T/A)G | G

Supplied buffer: basal

Effect of DNA methylation: enzyme activity is affected by both dcm and CG methylase

Reaction temperature: 30°C

More Information

• Source: Vibrio parahaemolyticus 1743-1
• Concentration: 4–12 units/µl
• Substrate for unit definition: lambda DNA
• Star activity: cleavage at alternative site(s) occurs in the presence of high concentrations of glycerol, DMSO, and at alkaline pH or low ionic strength.
• Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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