Thermo Fisher Scientific ATP6V1B2 Monoclonal Antibody (OTI1E11), TrueMAB
ATP6V1B2 단백질을 인식하는 Mouse IgG2a 단클론 항체로, WB 및 IHC(P)에서 검증됨. 인간 시료 반응성. Lyophilized 형태로 제공되며, PBS buffer에 8% trehalose 포함. 연구용으로 세포 내 산성화 관련 단백질 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E11 |
| Immunogen | Full length human recombinant protein of human ATP6V1B2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
ATP6V1B2 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme mediating acidification of eukaryotic intracellular organelles.
V-ATPase-dependent organelle acidification is essential for processes such as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.
The V1 domain of V-ATPase contains ATP catalytic sites and is composed of multiple subunits. ATP6V1B2 is one of two B subunit isoforms and is highly expressed in osteoclasts.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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