Thermo Fisher Scientific Chicken anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647

Applications

Tested Dilution

Western Blot (WB)

1:5000-1:10,000

Immunohistochemistry (IHC)

1-10 µg/mL

Immunocytochemistry (ICC/IF)

0.5-10 µg/mL

Product Specifications

Species Reactivity

Goat

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

View additional formats

• [Alexa Fluor 488 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 488/host/Chicken/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Chicken/)
• [AP](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/AP/host/Chicken/)
• [Biotin ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Biotin/host/Chicken/)
• [FITC](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/FITC/host/Chicken/)
• [HRP](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/HRP/host/Chicken/)
• [TRITC](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/TRITC/host/Chicken/)
• Request custom conjugation

Excitation/Emission Max

650/671 nm View spectra

Form

Liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient

RRID

AB_2535872

Target

IgG

Cross Adsorption

Against human, mouse and rabbit IgG prior to conjugation

Antibody Form

Whole Antibody

Product Specific Information

This Chicken anti-goat antibody reacts with IgG heavy chains and all classes of immunoglobulin light chains from goat. Chicken secondary antibodies have gained popularity because they demonstrate a lower level of nonspecific binding. Chicken antibodies lack a classic ″Fc″ domain and will not bind to protein A or protein G, nor will they bind to mammalian IgG Fc receptors. Fluorescence of this long-wavelength Alexa Fluor dye is not visible by looking through a conventional fluorescence microscope.

Product will be shipped at Room Temperature.

Target Information

Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.