Thermo Fisher Scientific Chicken anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488

Applications

Tested Dilution

Western Blot (WB)

1:2,500

Immunohistochemistry (IHC)

1-10 µg/mL

Immunocytochemistry (ICC/IF)

1 µg/mL

Product Specifications

Species Reactivity

Rat

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

View additional formats

• [Alexa Fluor 594 ](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Chicken/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 647/host/Chicken/)
• [AP](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/AP/host/Chicken/)
• [Biotin ](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/Biotin/host/Chicken/)
• [HRP](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/HRP/host/Chicken/)
• Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient

RRID

AB_2535873

Target

IgG

Cross Adsorption

Against human and rabbit IgG prior to conjugation

Antibody Form

Whole Antibody

Product Specific Information

Chicken anti-rat antibodies react with IgG heavy chains and all classes of immunoglobulin light chains from rat. Chicken secondary antibodies have gained popularity because they demonstrate a lower level of nonspecific binding. Chicken antibodies lack a classic ″Fc″ domain and will not bind to protein A or protein G, nor will they bind to mammalian IgG Fc receptors.

Product will be shipped at Room Temperature.

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.