
Thermo Fisher Scientific Chicken anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488
닭 유래 폴리클로날 항체로, 랫 IgG(H+L)에 특이적으로 반응하며 Alexa Fluor 488으로 표지됨. WB, IHC, ICC/IF에 적합. 낮은 비특이적 결합과 높은 신호 대비를 제공. 4°C 암조건 보관, 연구용 전용.
- 카탈로그번호
- A21470
- 판매단위
- pk
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Applications 및 권장 희석 배수
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,500 |
| Immunohistochemistry (IHC) | 1–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Chicken / IgY |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM Sodium Azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2535873 |
| Target | IgG |
| Cross Adsorption | Against human and rabbit IgG prior to conjugation |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 594
- Alexa Fluor 647
- AP (Alkaline Phosphatase)
- Biotin
- HRP (Horseradish Peroxidase)
- Custom conjugation available upon request
Product Specific Information
Chicken anti-rat antibodies react with IgG heavy chains and all classes of immunoglobulin light chains from rat.
Chicken secondary antibodies show reduced nonspecific binding because they lack the classical Fc domain and therefore do not bind to protein A, protein G, or mammalian IgG Fc receptors.
Product is shipped at room temperature.
Target Information
Anti-Rat secondary antibodies are affinity-purified with well-characterized specificity for rat immunoglobulins.
They are useful for detection, sorting, or purification of target molecules.
Secondary antibodies enhance detection versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to one primary antibody.
Typically, they are produced by immunizing the host with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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