Thermo Fisher Scientific TdT/DNA Nucleotidylexotransferase Monoclonal Antibody (TDT, 1393)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Antibody tested in direct ELISA (use at 2–4 µg/mL for coating).
• For immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.

Target Information

Terminal Deoxynucleotidyl Transferase (TdT) is a DNA polymerase located in the cell nucleus that catalyzes the polymerization of deoxynucleotides at the 3′ hydroxyl ends of oligo or polydeoxynucleotide initiators and functions without a template.
TdT is considered a highly specific marker for the diagnosis and classification of acute lymphoblastic lymphoma/leukemia.
The determination of TdT expression is valuable for differentiating histologically between lymphoblastic lymphoma and Burkitt’s lymphoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.