Thermo Fisher Scientific AMID Polyclonal Antibody
Human AMID 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot 및 IHC(P)에서 검증됨. 항원 친화 크로마토그래피로 정제된 액상 포맷이며, 1 mg/mL 농도. 단기 4°C, 장기 -20°C 보관 권장. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 1–43 of Human AMID |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546203 |
Product Specific Information
- Recommended positive controls: HepG2, Molt-4
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
The p53 tumor suppressor protein induces cell cycle arrest or apoptosis in response to cellular stresses.
PRG3 (p53-responsive gene 3) is induced specifically under p53-dependent apoptotic conditions in human colon cancer cells.
PRG3 has significant homology to bacterial oxidoreductases and the apoptosis-inducing factor (AIF).
Unlike AIF, PRG3 localizes in the cytoplasm and its ectopic expression induces apoptosis.
The PRG3 gene is thus a novel p53 target gene in a p53-dependent apoptosis pathway.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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